Voltammetric determination of neotame by using chitosan/nickelnanoparticles/multi walled carbon nanotubes biocomposite as a modifier

Abstract

A selective and simple biosensor was preparedby immobilizing chitosan/nickelnanoparticles/multi-walledcarbon nanotubes biocomposite on the glassy carbonelectrode surface for voltammetric quantification of neo-tame. The properties and morphology of the modifiedelectrode surfaces were characterized by scanning elec-tron microscope (SEM), energy dispersive X-ray analysis(EDX). Electro oxidation of neotame on this modifiedsurface was examined through cyclic voltammetry (CV)and square wave voltammetry (SWV) techniques. Thebiocomposite modified surface (Chi/NiNPs/MWCNTs/GCE) proposed in this study showed good electrocatalyticactivity for neotame with an improved voltammetric peakcurrent at 1.004 V, unlike the bare glassy carbon electrode(GCE) surface and several other modified surfaces.Under optimum conditions, Chi/NiNPs/MWCNTs/GCEgave linear SWV responses at the range of 2 M ~ 50 Mfor neotame with 0.84 M determination limit. Thisvoltammetric sensor was successfully employed for thequantification of neotame on food samples and showedlong-term stability, advanced voltammetric behavior, andgood repeatability. Selective, accurate, and precise deter-mination of neotame highlight the importance of thiselectrode in monitoring the control of food additives andensures attract a great deal of attention.