dc.contributor.author |
Onishchenko, Anatolii |
|
dc.contributor.author |
Myasoedov, Valeriy |
|
dc.contributor.author |
Nakonechna, Oksana |
|
dc.contributor.author |
Prokopyuk, Volodymyr |
|
dc.contributor.author |
Butov, Dmytro |
|
dc.contributor.author |
Kökbaş, Umut |
|
dc.contributor.author |
Klochkov, Vladimir |
|
dc.contributor.author |
Maksimchuk, Pavel |
|
dc.contributor.author |
Kavok, Nataliya |
|
dc.contributor.author |
Tkachenko, Anton |
|
dc.date.accessioned |
2022-06-15T14:02:11Z |
|
dc.date.available |
2022-06-15T14:02:11Z |
|
dc.date.issued |
2021-08-13 |
|
dc.identifier.uri |
https://link.springer.com/article/10.1007/s12011-021-02867-z |
|
dc.identifier.uri |
http://hdl.handle.net/20.500.11787/6968 |
|
dc.description.abstract |
Nanoparticles (NPs) have been reported to be promising enhancement agents for radiation therapy. The aim of the study was to assess the cytotoxicity of UV non-treated and UV pretreated GdYVO4:Eu3+ nanoparticles against erythrocytes and leukocytes by detecting eryptosis and reactive oxygen species (ROS) generation. Levels of intracellular ROS in erythrocytes and leukocytes using a ROS-sensitive dye 2ʹ,7ʹ-dichlorodihydrofuorescein diacetate (H2DCFDA), as well as eryptosis rate utilizing annexin V staining, following direct exposure to UV-activated and nonactivated NPs were detected by fow cytometry. Blood cells were collected from 9 intact WAG rats. Neither the UV light-untreated GdYVO4:Eu3+ NPs nor the treated ones promoted eryptosis and ROS generation in erythrocytes. Low concentrations of UV light-untreated NPs did not induce
oxidative stress in leukocytes, evidenced by unafected intracellular ROS levels. UV light treatment grants prooxidant properties to NPs, confrmed by NP-induced ROS overproduction in leukocytes. High concentrations of both UV light-treated and untreated NPs altered the redox state of leukocytes. UV light treatment imparts prooxidant properties to GdYVO4:Eu3+ NPs, making them promising radiosensitizing agents in cancer radiation therapy. |
tr_TR |
dc.description.sponsorship |
Ukrayna Sağlık Bakanlığı 0121U110920 |
tr_TR |
dc.language.iso |
eng |
tr_TR |
dc.publisher |
Springer |
tr_TR |
dc.rights |
info:eu-repo/semantics/openAccess |
tr_TR |
dc.subject |
Eryptosis |
tr_TR |
dc.subject |
2ʹ,7ʹ-dichlorodihydrofuorescein diacetate |
tr_TR |
dc.subject |
Flow cytometry |
tr_TR |
dc.subject |
Annexin V |
tr_TR |
dc.subject |
Nanomaterials |
tr_TR |
dc.subject |
Oxidative stress |
tr_TR |
dc.title |
UV Light-Activated GdYVO4:Eu3+ nanoparticles ınduce reactive oxygen species generation in leukocytes without affecting erythrocytes in vitro |
tr_TR |
dc.type |
article |
tr_TR |
dc.relation.journal |
Biological Trace Element Research |
tr_TR |
dc.contributor.department |
Nevşehir Hacı Bektaş Veli Üniversitesi/diş hekimliği fakültesi/temel bilimler bölümü/temel tıp bilimleri anabilim dalı |
tr_TR |
dc.contributor.authorID |
158848 |
tr_TR |
dc.identifier.volume |
200 |
tr_TR |
dc.identifier.startpage |
2777 |
tr_TR |
dc.identifier.endpage |
2792 |
tr_TR |