UV Light-Activated GdYVO4:Eu3+ nanoparticles ınduce reactive oxygen species generation in leukocytes without affecting erythrocytes in vitro

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dc.contributor.author Onishchenko, Anatolii
dc.contributor.author Myasoedov, Valeriy
dc.contributor.author Nakonechna, Oksana
dc.contributor.author Prokopyuk, Volodymyr
dc.contributor.author Butov, Dmytro
dc.contributor.author Kökbaş, Umut
dc.contributor.author Klochkov, Vladimir
dc.contributor.author Maksimchuk, Pavel
dc.contributor.author Kavok, Nataliya
dc.contributor.author Tkachenko, Anton
dc.date.accessioned 2022-06-15T14:02:11Z
dc.date.available 2022-06-15T14:02:11Z
dc.date.issued 2021-08-13
dc.identifier.uri https://link.springer.com/article/10.1007/s12011-021-02867-z
dc.identifier.uri http://hdl.handle.net/20.500.11787/6968
dc.description.abstract Nanoparticles (NPs) have been reported to be promising enhancement agents for radiation therapy. The aim of the study was to assess the cytotoxicity of UV non-treated and UV pretreated GdYVO4:Eu3+ nanoparticles against erythrocytes and leukocytes by detecting eryptosis and reactive oxygen species (ROS) generation. Levels of intracellular ROS in erythrocytes and leukocytes using a ROS-sensitive dye 2ʹ,7ʹ-dichlorodihydrofuorescein diacetate (H2DCFDA), as well as eryptosis rate utilizing annexin V staining, following direct exposure to UV-activated and nonactivated NPs were detected by fow cytometry. Blood cells were collected from 9 intact WAG rats. Neither the UV light-untreated GdYVO4:Eu3+ NPs nor the treated ones promoted eryptosis and ROS generation in erythrocytes. Low concentrations of UV light-untreated NPs did not induce oxidative stress in leukocytes, evidenced by unafected intracellular ROS levels. UV light treatment grants prooxidant properties to NPs, confrmed by NP-induced ROS overproduction in leukocytes. High concentrations of both UV light-treated and untreated NPs altered the redox state of leukocytes. UV light treatment imparts prooxidant properties to GdYVO4:Eu3+ NPs, making them promising radiosensitizing agents in cancer radiation therapy. tr_TR
dc.description.sponsorship Ukrayna Sağlık Bakanlığı 0121U110920 tr_TR
dc.language.iso eng tr_TR
dc.publisher Springer tr_TR
dc.rights info:eu-repo/semantics/openAccess tr_TR
dc.subject Eryptosis tr_TR
dc.subject 2ʹ,7ʹ-dichlorodihydrofuorescein diacetate tr_TR
dc.subject Flow cytometry tr_TR
dc.subject Annexin V tr_TR
dc.subject Nanomaterials tr_TR
dc.subject Oxidative stress tr_TR
dc.title UV Light-Activated GdYVO4:Eu3+ nanoparticles ınduce reactive oxygen species generation in leukocytes without affecting erythrocytes in vitro tr_TR
dc.type article tr_TR
dc.relation.journal Biological Trace Element Research tr_TR
dc.contributor.department Nevşehir Hacı Bektaş Veli Üniversitesi/diş hekimliği fakültesi/temel bilimler bölümü/temel tıp bilimleri anabilim dalı tr_TR
dc.contributor.authorID 158848 tr_TR
dc.identifier.volume 200 tr_TR
dc.identifier.startpage 2777 tr_TR
dc.identifier.endpage 2792 tr_TR


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